Jackline Ngonyani said any MPs found not to have been circumcised should be required to undergo the procedure.
Her suggestion divided opinion among her colleagues.
HIV is seen as a major threat to public health in Tanzania. Around 70 percentof the male population is circumcised.
Ms Ngonyani made the comments during a debate in parliament about how to curb the spread of HIV in the country.
Her suggestion was backed by MP Joseph Selasini.
In neighbouring Kenya, some top politicians voluntarily submitted to the procedure in 2008 as a way of encouraging men from their communities to do the same.
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